The Largest VC Firms in Czech Republic
Leading venture capital firms in Czech Republic, ranked by reported assets under management (AUM). Discover the most active local VCs and unlock full profiles to reach out.
Capital Landscape
Czech Republic sits at an interesting position within Eastern Europe's venture ecosystem. The country hosts one of the region's more developed startup scenes, anchored by Prague, yet the concentration of large, institutionally managed VC funds remains relatively modest compared to Western European hubs like Berlin, Stockholm, or Amsterdam. The majority of AUM managed in the Czech market is held by a small number of funds, many of which operate regionally across Central and Eastern Europe (CEE) rather than focusing exclusively on Czech deal flow.
Fund sizes in Czech Republic tend to cluster in the lower-to-mid range by European standards. Funds managing between €50 million and €200 million represent the dominant tier, with only a handful of managers approaching or exceeding the €300 million mark. This reflects the broader CEE pattern: limited partners, including European Investment Fund-backed vehicles and domestic institutional capital, have historically committed to smaller pools than their Western counterparts, though successive fund vintages have gradually grown in size.
Compared to regional peers, Czech Republic punches above its weight in deal volume relative to GDP, partly because Prague has cultivated a dense concentration of technical talent and a generation of founders who have exited and reinvested as angels or GPs. Poland commands a larger absolute pool of VC capital due to its market size, while Estonia has a disproportionate number of unicorn-per-capita stories. Czech Republic sits between these poles: more mature infrastructure than most of the Balkans, less total capital than Warsaw or Budapest at the fund level. EU structural funds have continued to play a meaningful role in capitalizing local vehicles, which affects return expectations and how funds are governed.
Notable Fund Archetypes
These funds invest across Czech Republic and neighboring CEE markets without restricting themselves to a single vertical. They typically enter at seed or Series A and follow on through later rounds, deploying capital across software, fintech, and digital services depending on where deal quality is strongest in a given cycle.
Backed partly by European public capital programs, these funds target university spinouts and R&D-intensive companies coming out of Czech technical institutions. Typical focus areas include advanced materials, cybersecurity, aerospace components, and industrial automation, with longer hold periods than software-focused peers.
Several funds operating in the Czech market were capitalized with support from the European Investment Fund or domestic state-linked entities. They carry a mandate to support early-stage Czech companies specifically, which constrains geographic scope but also creates more predictable deal access for Prague-based founders.
Larger Czech industrial groups and a handful of international corporations have established or backed CVC vehicles that invest in startups relevant to their core business. Strategic fit matters as much as financial return, and portfolio companies often gain distribution or procurement relationships alongside capital.
A smaller but growing cohort of funds targets Czech and CEE companies that have already demonstrated product-market fit and are scaling into Western European or North American markets. Ticket sizes are larger, diligence timelines are longer, and these funds frequently co-invest with international growth-stage investors entering the region.
How to Approach These Funds
Founders approaching larger VC funds in Czech Republic should understand that most of these managers think in regional terms, not purely national ones. A fund based in Prague is almost certainly evaluating deals across Poland, Slovakia, Romania, and the Baltics alongside Czech opportunities. This means your pitch needs to articulate why your company is positioned to win in CEE broadly, or why the Czech market is a credible launchpad for a larger European expansion, rather than treating the domestic market as the endpoint.
Warm introductions carry significant weight at this tier. The Czech VC community is small and relationship-dense. A cold inbound through a fund's website is rarely how a meaningful conversation starts. The most reliable paths to a first partner meeting run through portfolio founders who can vouch for you, co-investors the fund already works with, or ecosystem connectors inside accelerators and incubators that have established credibility with specific GPs. Attending Prague-based startup events where fund partners are genuinely present (not just speaking on panels) gives you a lower-stakes opportunity to make a human impression before a formal pitch.
At the larger AUM tier, funds in this market are increasingly metrics-oriented at Series A and beyond. Showing early traction through revenue, user retention data, or clear enterprise pipeline matters more than narrative alone. Funds backed by institutional LPs face reporting obligations that push them toward deals with legible growth trajectories. If you are pre-revenue, your technical differentiation and team pedigree need to be unusually strong to clear the bar.
Stage fit is something founders frequently misjudge. Some funds that present themselves as multi-stage actually deploy the bulk of their capital at one specific point in the funnel. Ask directly about average initial check size and the number of new investments made per year rather than relying on a fund's public positioning.
Top VC firms in Czech Republic
231 VC firms with reported AUM. Sign up to unlock full profiles.
| # | VC Firm | AUM |
|---|---|---|
| 1 | EQT Holdings AB EQT is a global investment organization focused on active ownership and thematic investing across private capital, infrastructure, and real estate, from early-stage ventures to large-cap buyouts. | |
| 2 |  Silicon Valley Bank Silicon Valley Bank (SVB) is a specialized financial partner for the innovation economy, serving startups from pre-seed to IPO, as well as venture capital and private equity investors globally. | |
| 3 | Lendable Lendable is a technology-enabled investment platform providing debt and financing solutions to high-growth fintech companies and impact alternatives in emerging and frontier markets. | |
| 4 |  Novo Holdings Novo Holdings A/S is the holding and investment company responsible for managing the assets of the Novo Nordisk Foundation, one of the world's largest enterprise foundations. Established in 1999, the company is headquartered in Copenhagen, Denmark, with offices in London, San Francisco, Boston, Singapore, and Shanghai. As of 2024, Novo Holdings manages total assets of DKK 1,060 billion (€142 billion).
The company's primary objective is to improve people's health and the sustainability of society and the planet by generating attractive long-term returns on the assets of the Novo Nordisk Foundation.
To achieve this, Novo Holdings invests in life science companies at all stages of development and manages a diversified portfolio that includes equities, bonds, real estate, infrastructure assets, and private equity investments.
In 2024, Novo Holdings reported record total income and investment returns of DKK 60 billion (€8.0 billion), driven by strong performances from its investment portfolio and the Novo Group companies.
A significant recent development was the completion of a $16.5 billion acquisition of Catalent, a contract drug manufacturer, in December 2024. This strategic move aims to bolster the production of Wegovy, a popular weight-loss drug, to meet increasing demand.
Novo Holdings' investment areas include life science investments, capital investments, and planetary health investments, with a focus on regions such as North America, Europe, and Asia.
The company's guiding principles emphasize high performance with respect and responsibility, an ambitious strategy focused on return-driven investments, and excellence in operations, including talent development and strong governance. | |
| 5 |  Advent Life Sciences Advent Life Sciences is one of the leading trans-Atlantic venture investors focused on building innovative Life Sciences businesses in the UK, Europe and the USA. We are a highly experienced team with a long-standing track record of entrepreneurial and inve stment success through turning break-through science into approved medicines or medical products. We start and invest in early and mid-stage companies with a first-in-class or best-in-class approach. Our investments are focussed in new drug discovery - small molecules, biologics and new modalities - med tech, enabling technologies and vaccines. | |
| 6 |  Criteria Venture Tech Criteria Venture Tech is a multi-stage Venture Capital fund, committed to propelling the future of technology. Our mission is to partner with visionary companies that are at the forefront of technological innovation.
We invest in tech companies in Europe and North America, with special focus in Spain and Portugal. We invest agnostically across various stages and verticals, with a specific focus on B2B SaaS and Deeptech, paying particular attention to key areas such as Software Infrastructure, Enterprise Data and Automation, Frontier Tech and Cybersecurity, offering evergreen support until achieving meaningful impact. We believe in creating long-term economic and social value through technological breakthroughs.
Criteria Venture Tech is part of Caixa Capital Risc and the CriteriaCaixa group, Spain's largest investment holding with over €25 billion in assets under management. | |
| 7 |  J&T INVESTICNÍ SPOLECNOST, a.s. J&T INVESTICNÍ SPOLECNOST, a.s. is a venture capital arm of J&T BANKA, a.s. specializing in growth capital and startups in seed and early stages in the Fintech sector. While not sector limited at all, its investments primarily focus on opportunities in IoT/IoE and smart city, transport and energy, retail and agriculture, information technologies including Big Data analytics and security, finance including fintech and mobile payments, education including EdTech and Educational tools, healthcare including medtech and diagnostic services, and Internet including software, e-commerce, and gaming, sensors, data analytics and prediction. The firm invests in the CEE and SEE regions, Nordics and Baltics. It seeks to invest up to €2.5 million ($2.69 million) in companies. J&T INVESTICNÍ SPOLECNOST, a.s. was founded in 1997 and is based in Prague, Czech Republic. | |
| 8 |  TenOneTen Ventures TenOneTen Ventures is a Los Angeles-based venture capital firm that invests early in technical teams transforming major industries like healthcare, logistics, and manufacturing using AI and data. | |
| 9 |  BAM Ventures BAM Ventures is a Los Angeles-based venture capital firm founded in 2014 by Brian Lee and Richard Jun, both seasoned entrepreneurs with extensive experience in building and scaling companies. The firm focuses on early-stage investments, targeting resilient founders who have the vision to launch and scale innovative technologies, products, and services that connect with consumers and transform commerce. BAM Ventures emphasizes a hands-on approach, offering not only capital but also time, experience, and relationships to support entrepreneurs in building companies that matter.
Their portfolio includes notable companies such as Honey (acquired by PayPal), Tala, Wondery (acquired by Amazon), Flow, Rael, and Modern Animal. | |
| 10 |  Lightspeed Venture Partners Lightspeed Venture Partners is a multi-stage venture capital firm focused on accelerating disruptive innovations and trends in the Enterprise, Consumer, and Health sectors. Over the past two decades, the Lightspeed team has backed hundreds of entrepreneurs and helped build more than 400 companies globally, including Snap, Affirm, Nest, Nutanix, AppDynamics, MuleSoft, OYO, Guardant, and GrubHub. Lightspeed and its affiliates currently manage $10.5B across the global Lightspeed platform, with investment professionals and advisors in Silicon Valley, Israel, India, China, Southeast Asia and Europe. | |
| 11 |  Penta Investments Ltd. Penta is a Central European investment group established in 1994. Today, it focuses on long-term investments into healthcare, financial services, manufacturing, retail, media and real estate development. Penta is one of the largest employers in the region and provides jobs to more than 44,000 people. The Group operates in more than 10 European countries and has offices in Prague, Bratislava and Warsaw. | |
| 12 |  SWEN Capital Partners SWEN Capital Partners is a leading responsible investor in unlisted assets with over €9.5 billion under management. They focus on sustainable finance, supporting environmental and societal growth across private equity, real assets (infrastructure), and private debt. | |
| 13 |  Invus Invus is an evergreen equity investment firm founded in 1985, managing over $10 billion in capital. With offices in New York, Paris, and Hong Kong, Invus focuses on empowering owner-managers to transform their industries by providing the freedom to take risks, the capital to reach their goals, and the strategic advice to navigate uncharted waters. The firm emphasizes a long-term investment horizon, aiming for exponential, sustainable value creation through building sustainable competitive advantages and innovating to fulfill real customer needs.
Invus operates with a unique structure that allows flexibility in the types of companies and situations they engage with, fully aligning their incentives with their partners and demonstrating a long-term commitment to see through any challenges along the way. Since its inception, Invus has been successfully empowering owner-managers, with its source of capital being a European family group through their Artal investment vehicle. This evergreen structure has enabled Invus to compound its initial pool of capital over four decades, growing to over $10 billion even after distributing billions to shareholders. | |
| 14 | a16z crypto a16z crypto is a venture capital fund that invests in crypto and blockchain startups. It recently raised a new $2.2 billion fund, bringing its total raised to date to $9.8 billion. | |
| 15 |  Perceptive Advisors Perceptive is focused on supporting the progress of the life sciences industry by identifying opportunities and directing financial resources to the most promising technologies in healthcare.The information presented in this page is included to enable professional networking by Perceptive Advisor’s employees and should not be used for any other purpose. As such, Perceptive Advisor’s use of LinkedIn is not an offer to, or solicitation of, any potential clients or investors for the provision of investment management, advisory or any other related services. No information posted by Perceptive Advisors should be construed as investment advice, or as an offer to sell, or a solicitation of an offer to buy, any security or other instrument. | |
| 16 | Norwest Venture Partners Norwest Venture Partners is a leading Silicon-Valley based venture capital and growth equity investment firm managing $7.5 billion in capital. Since our inception, we have invested in more than 600 companies. The firm invests in early to late stage companies across a wide range of sectors with a focus on consumer, enterprise, and healthcare.We offer a deep network of connections, operating experience, and a wide range of impactful services to help CEOs and founders advance on their journey.We are proud to have backed entrepreneurs at Apigee, Casper, FireEye, Jet, Kendra Scott, and Udemy among others.Norwest has offices in Palo Alto and San Francisco, with subsidiaries in Mumbai and Bengaluru, India and Herzelia, Israel. | |
| 17 |  Redstone Redstone is a Berlin-based Venture Capital firm managing multiple VC Funds and working with corporate venture funds. We reinvented corporate Venture Capital through our VC-as-a-Service model, which connects industry leaders with ambitious founders.Together with our industry partners, we activate corporate capital, knowledge and network to support our entrepreneurs in growing their businesses. Additionally to our investment service we provide tailored intelligence that empowers partners to make better-informed strategic decisions and stay informed about future digital trends.Redstone is run by experienced entrepreneurs and investors with deep roots in the German and European technology ecosystem. Our founding partners have built and advised some of Europe's largest technology companies over the last two decades.With globally operating investment and data analytics teams, we strive to find and support the most ambitious ventures in sectors and stages that match our funds' investment theses. As a trusted partner, we pride our self on fast and transparent communication both on the startup and the investor side. | |
| 18 |  Vertex Venture Holdings Vertex is a global network of venture capital funds. The Vertex network of funds invests in early stage opportunities in technology through Vertex Ventures and healthcare through Vertex Ventures HC, and growth stage opportunities through Vertex Growth. Each of the Vertex Ventures funds in Israel, USA, China, and SEA & India operate independently, with separate local teams that raise and manage respective funds independently.Vertex Holdings is an anchor investor in each of the Vertex funds, namely: Vertex Ventures China, Vertex Ventures USA, Vertex Ventures Israel, Vertex Ventures SEA & India, Vertex Ventures HC, and Vertex Growth. Vertex Holdings is owned by Temasek Holdings. | |
| 19 |  Canaan Partners Canaan is an early-stage venture capital firm that invests in visionaries with transformative ideas. While we love speedy success, we're acutely aware that greatness comes in many forms and at different times. That's why we're not afraid to break the mold to bring something spectacular to life.
Our approach is personal.
We don’t believe in a one-size-fits-all approach, but there are a few absolutes intrinsic to who we are and how we work. And these values weren’t contrived out of thin air, they’re hard earned over the past 30 years and counting.
01
High performance, low ego.
The old adage “actions speak louder than words” rings true within Canaan’s walls, and in our interactions with companies out in the wild. Words like "understated" and "effective" are often used to describe our partners, and that’s fine by us. We’ll leave the bragging to the others.
“Seasoned investors can be very arrogant, but Canaan is no BS, unassuming, and humble—no hysteria, no angst.”
— Michael Gilman, CEO Arrakis
02
People are more than statistics.
A diverse team means we bring varied perspectives to the table. And that table welcomes out-of-the-box thinking, spirited debate, and people from all walks of life. It’s our individual backgrounds and interests that inform our collective approach, which spans industries, generations, and coasts. In short, our diversity is who we are and what we do.
03
We ask for help, and so should you.
We firmly believe that two heads are better than one. So while our investors thrive in 1:1 partnerships, our companies also have the support of the entire Canaan team whenever, wherever. Recruiting your executive team? We’ll mobilize our full network. Need operational advice to bring a product to market? We’ll connect you with the right partner or colleague. Our resources are yours.
“Even if Canaan hadn’t funded us, just going through the process with them – the discussions we had and the questions they asked – already made us a better company. And that’s amazing.”
— Julie Wainwright, CEO The RealReal
04
The Golden Rule still applies.
We treat you like we treat ourselves—with respect, transparency, and honesty—even if it’s sometimes not what you want to hear. It’s this approach that makes our portfolio companies more like family, because we’re not afraid to get in the weeds and help solve big hairy problems. In fact, it’s what we live for. We’re acutely aware that our success hinges on your success, so we do what it takes.
“We made big strategic shifts as a company and worked closely with Maha through all of them. Canaan is really smart about the way they get involved and how they support you through good times and bad.”
— Kevin Chou, former CEO Kabam
05
We're in the know, not in the way.
We’d be lying if we said we weren’t hands-on, but we also know when to step back and let you soar. Our goal is to add value to your process, and help you grow in ways you couldn’t alone. That’s why you’ll never see us booking meetings just to check a box or “see how are things are going.” We’ll already know, because that’s the kind of relationship we have with our companies.
Balance, diversity and passion are at the core of who we are. Meet the hardworking people who defy convention, shatter glass ceilings, and take companies to the top — all with a unique style and a healthy dose of humor. | |
| 20 |  8VC 8VC is a technology and life sciences venture capital firm that builds and invests in transformative companies across various sectors, including life sciences, healthcare, manufacturing, enterprise, logistics, and defense. Founded in 2015 by Joe Lonsdale, a co-founder of Palantir Technologies, the firm is headquartered in Austin, Texas, and manages over $6 billion in committed capital. 8VC's mission is to "fix a broken world" by partnering with entrepreneurs to develop innovative solutions to complex global challenges.
The firm invests at all stages of a company's lifecycle, from seed to growth, and also builds companies through its 8VC Build program. Notable portfolio companies include Palantir Technologies, Anduril Industries, and Guardant Health. 8VC's investment philosophy emphasizes long-term value creation and societal impact, focusing on sectors that have the potential to drive significant positive change.
The firm's team comprises experienced professionals with diverse backgrounds in technology, finance, and entrepreneurship, enabling them to provide comprehensive support to their portfolio companies. | |
| 21 |  Forbion Forbion is a global venture capital firm dedicated to advancing groundbreaking biotech innovations and sustainable solutions in human health and the bioeconomy, managing over €5bn across 11 funds. | |
| 22 |  Primavera Capital Group Primavera Capital Group is a premier Asia-Pacific-based global investment firm that seeks to develop trusted, lasting partnerships with high-potential growth companies to promote entrepreneurship and fuel innovation. | |
| 23 |  Northgate Capital Group For more 25 years, Northgate has been partnering with leading venture capital companies and managers to provide long-term value and growth for entrepreneurs and clients through direct investments, primaries, and secondaries. | |
| 24 |  Polaris Partners Polaris Partners is a venture capital firm that has collaborated for nearly three decades with talented biotech and healthcare entrepreneurs to build transformational companies. | |
| 25 |  Sofinnova Partners Sofinnova Partners is a leading European venture capital firm in life sciences, specializing in healthcare and sustainability. Based in Paris, London and Milan, the firm brings together a team of professionals from all over the world with strong scientific, medical and business expertise. Sofinnova Partners is a hands-on company builder across the entire value chain of life sciences investments, from seed to later-stage. The firm actively partners with ambitious entrepreneurs as a lead or cornerstone investor to develop transformative innovations that have the potential to positively impact our collective future.Founded in 1972, Sofinnova Partners is a deeply-established venture capital firm in Europe, with 50 years of experience backing over 500 companies and creating market leaders around the globe. Today, Sofinnova Partners has over €4 billion under management. For more information, please visit: www.sofinnovapartners.com |
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